Journal of Neurophysiology Direct Inhibition of Ih by Analgesic Loperamide in Rat DRG Neurons
نویسندگان
چکیده
Direct Inhibition of Ih by Analgesic Loperamide in Rat DRG Neurons Dmitry V. Vasilyev, Qin Shan, Yan Lee, Scott C. Mayer, Mark R. Bowlby, Brian W. Strassle, Edward J. Kaftan, Kathryn E. Rogers, John Dunlop Discovery Neuroscience and Chemical and Screening Sciences, Wyeth Research, CN 8000, Princeton, NJ 08543-8000 Page 1 of 37 Articles in PresS. J Neurophysiol (March 28, 2007). doi:10.1152/jn.00841.2006
منابع مشابه
Direct inhibition of Ih by analgesic loperamide in rat DRG neurons.
Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are responsible for the functional hyperpolarization-activated current (I(h)) in dorsal root ganglion (DRG) neurons, playing an important role in pain processing. We found that the known analgesic loperamide inhibited I(h) channels in rat DRG neurons. Loperamide blocked I(h) in a concentration-dependent manner, with an IC(50) = ...
متن کاملRole of a voltage-sensitive calcium channel blocker on inhibition of apoptosis in sensory neurons of cultured dorsal root ganglia in adult rat
Introduction: Under pathological conditions, abnormal increase in intracellular calcium concentrations is believed to induce cell death. In the present study, a voltage-sensitive calcium channel blocker (loperamide hydrochloride) was used to investigate its role in inhibition of apoptosis in sensory neurons of cultured spinal dorsal root ganglia (DRG). Methods: L5 DRG from adult rats were di...
متن کاملEffect of intracellular dialysis of ATP on the hyperpolarization-activated cation current in rat dorsal root ganglion neurons.
The mechanism of the effect of intracellular ATP on the hyperpolarization-activated non-selective cation current (Ih) in rat dorsal root ganglion neurons was investigated using a whole cell voltage-clamp technique. Under voltage-clamp conditions, Ih was activated by hyperpolarizing pulses raised to a voltage of between -70 and -130 mV. The activation curve of Ih in rat dorsal root ganglion (DRG...
متن کاملInhibition of a hyperpolarization-activated current by clonidine in rat dorsal root ganglion neurons.
Whole cell voltage- and current-clamp recordings were carried out to investigate the effects of clonidine, an alpha 2-adrenoceptor agonist, in L4 and L5 dorsal root ganglion (DRG) neurons of the rat. In voltage-clamp mode, application of 20 microM clonidine reversibly reduced the inward current evoked by hyperpolarizing voltage steps. The "clonidine-sensitive current" was obtained by subtractin...
متن کاملMorphological Identification of Cell Death in Dorsal Root Ganglion Neurons Following Peripheral Nerve injury and repair in adult rat
Background: Axotomy causes sensory neuronal loss. Reconnection of proximal and distal nerve ends by surgical repair improves neuronal survival. It is important to know the morphology of primary sensory neurons after the surgical repair of their peripheral processes. Methods: Animals (male Wistar rats) were exposed to models of sciatic nerve transection, direct epineurial suture repair of sciati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2007